Solution NMR Characterization of an Unusual Distal H-Bond Network in the Active Site of the Cyanide-Inhibited Human Heme Oxygenase Complex of the Symmetric Substrate 2,4-Dimethyldeuterohemin. Li, Y., Syvitiski, R., Auclair, K., Wilks, A., Ortiz de Montellano, P. R., and La Mar, G. N. (2002) J. Biol. Chem., in press.
1H NMR Detection of Immobilized Water Molecules within a Strong Hydrogen-Bonding Distal Network in Substrate-bound Human Heme Oxygenase. Syvitski, R. T., Li, Y., Auclair, K., Ortiz de Montellano, P. R., and La Mar, G. N. (2002) Submitted for publication.
The Genome of Saccharomyces cerevisiae does not Include a Heme Oxygenase. Cloning and Expression of a Novel Heme Protein. Auclair, K., Moënne-Loccoz, P., and Ortiz de Montellano, P. R. (2002) Submitted for publication.
Solution 1H NMR of the Active Site of Substrate-Bound, Cyanide-Inhibited, Human Heme Oxygenase: Comparison to the Crystal Structure of the Water-ligated Form. La Mar, G. N., Asokan, A., Espiritu, B., Yeh, D. C., Auclair, K., and Ortiz de Montellano, P. R. (2001) J. Biol. Chem. 276, 15676-15687. HTML, PDF
Disruption of an Active Site Hydrogen bond converts Human Heme Oxygenase-1 into a Peroxidase. Lightning, L.K., Huang, H., Moenne-Loccoz, P., Loehr, T.M., Schuller, D.J., Poulos, T.L., and Ortiz de Montellano, P.R. (2001) J. Biol. Chem. 276, 10612-10619. HTML, PDF
Replacement of the Distal Glycine-139 Transforms Human Heme Oxygenase-1 into a Peroxidase. Liu, Y., Lightning, L.K., Huang, H.-W., Moenne-Loccoz, P., Schuller, D.J. Loehr, T.M., Poulos, T.L., and Ortiz de Montellano, P.R. (2000) J. Biol. Chem. 275, 34501-34507. HTML, PDF
Reaction Intermediates and Single Turnover Rate Constants for the Oxidation of Heme by Human Heme Oxygenase-1. Liu, Y., and Ortiz de Montellano, P.R. (2000) J. Biol. Chem. 275, 5297-5307. HTML, PDF
Assignment of the Heme Axial Ligand(s) for the Ferric Myoglobin (H93G) and Heme Oxygenase (H25A) Cavity Mutants as Oxygen Donors Using Magnetic Circular Dichroism. Pond, A. E., Roach, M. P., Sono, M., Rux A. H., Franzen, S., Hu, R., Ramser-Thomas, M., Wilks, A., Ikeda-Saito, M., Ortiz de Montellano, P. R., Woodruff, W. H., Boxer, S. G., and Dawson, J. H. (1999) Biochemistry 38, 7601-7608. HTML
Crystal Structure of Human Heme Oxygenase-1. Schuller, D., Wilks, A., Ortiz de Montellano, P.R., and Poulos, T. (1999) Nature Struct. Biol. 6, 860-867.
Replacement of the Proximal Histidine Iron Ligand by a Cysteine or Tyrosine Converts Heme Oxygenase to an Oxidase. Liu, Y., Moënne-Loccoz, P., Hildebrand, D., Wilks, A., Loehr, T. M., Mauk, A. G., and Ortiz de Montellano, P. R. (1999) Biochemistry 38, 3733-3743. HTML PDF
Synthesis of Photoaffinity Probes for Heme-Containing Proteins. Tschirret-Guth, R. A., and Ortiz de Montellano, P. R. (1998) J. Org. Chem. HTML PDF
Solution 1H NMR Investigation of the Molecular and Electronic Structure of the Active Site of Substrate-Bound Human Heme Oxygenase: the Nature of the Distal Hydrogen Bond Donor to Bound Ligands. Gorst, C. M., Wilks, A., Yeh, D. C., Ortiz de Montellano, P. R., and La Mar, G. N. (1998) J. Am. Chem. Soc. 120, 8875-8884. HTML PDF
Crystallization of Recombinant Human Heme Oxygenase-1. Schuller, D., Wilks, A., Ortiz de Montellano, P. R., and Poulos, T. L. (1998) Protein Science 7, 1836-1838.
Heme Oxygenase Active-Site Residues Identified by Heme-Protein Cross-linking during Reduction of CBrCl3. Wilks, A., Medzihradsky, K., and Ortiz de Montellano, P. R. (1998) Biochemistry 37, 2889-2896. HTML PDF
Oxidation of a-meso-Formylmesoheme by Heme Oxygenase. Electronic control of the reaction regiospecificity. Torpey, J., and Ortiz de Montellano, P. R. (1997) J. Biol. Chem. 272, 22008-14. HTML PDF
Autocatalytic Processing of Heme by Lactoperoxidase Produces the Native Protein-Bound Prosthetic Group. DePillis, G. D., Ozaki, S., Kuo, J. M., Maltby, D. A., and Ortiz de Montellano, P. R. (1997) J. Biol. Chem. 272, 8857-8860. HTML PDF
Heme Oxygenase-1, Intermediates in Verdoheme Formation and the Requirement for Reduction Equivalents. Liu, Y., Moënne-Loccoz, P., Loehr, T., and Ortiz de Montellano, P. R. (1997) J. Biol. Chem. 272, 6909-6917. HTML PDF
Oxidation of the meso-Methylmesoheme Regioisomers by Heme Oxygenase. Electronic Control of the Reaction Regiospecificity. Torpey, J., and Ortiz de Montellano, P. R. (1996) J. Biol. Chem. 271, 26067-26073. HTML PDF
Oxidation of an a-meso-Methyl-Substituted Heme to an a-Biliverdin by Heme Oxygenase. A Novel Heme Cleavage Reaction. Torpey, J., Lee, D. A., Smith, K. M., and Ortiz de Montellano, P. R. (1996) J. Am. Chem. Soc. 118, 9172-9173. HTML PDF
Ligation of the Iron in Heme Oxygenase: X-Ray Absorption, Electronic Absorption, and Magnetic Circular Dichroism Studies, Hawkins, B. K., Wilks, A., Powers, L. S., Ortiz de Montellano, P. R., and Dawson, J. H. (1996) Bio chim. Biophys. Acta. 1295, 165-73.
Heme Oxygenase (HO-1): His-132 Stabilizes a Distal Water Ligand and Assists Catalysis. Wilks, A., Ortiz de Montellano, P. R., Sun, J., and Loehr, T. M. (1996) Biochemistry 35, 930-936. HTML PDF
Synthesis and Assignment of the Four Meso-Methylmesoporphyrin IX Regioisomers. Torpey, J. W., and Ortiz de Montellano, P. R. (1995) J. Org. Chem. 60, 2195-2199.
Expression and Characterization of Truncated Human Heme Oxygenase (hHO-1) and a Fusion Protein of hHO-1 with Human Cytochrome P450 Reductase. Wilks, A., Black, S. M., Miller, W. L., and Ortiz de Montellano, P. R. (1995) Biochemistry 34, 4421-4427.
Heme Oxygenase His-25->Ala Mutant: Replacement of the Proximal Histidine Iron Ligand by Exogenous Bases Restores Catalytic Activity. Wilks, A., Sun, J., Loehr, T. M., and Ortiz de Montellano, P. R. (1995) J. Am. Chem. Soc. 117, 2925-2926.
Identification of Histidine-25 as the Heme Ligand in Human Liver Heme Oxygenase, Sun, J., Loehr, T. M., Wilks, A., and Ortiz de Montellano, P. R. (1994) Biochemistry 33, 13734-13740.
Heme Oxygenase (HO-1): Evidence for Electrophilic Addition of Oxygen to the Porphyrin Ring in the Formation of a-meso-Hydroxyheme. Wilks, A., Torpey, J., and Ortiz de Montellano, P. R. (1994) J. Biol. Chem. 269, 29553-29556. Abstract
Proton NMR Investigation of Substrate-Bound Heme Oxygenase: Evidence for Electronic and Steric Contributions to Stereoselective Heme Cleavage. Hernández, G., Wilks, A., Paolesse, R., Smith, K. M., Ortiz de Montellano, P. R., and La Mar, G. N. (1994) Biochemistry 33, 6631-6641.
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