Publications, 1994 - present

Publications for the Years 1994-2008


Cytochrome P450
Heme Oxygenase
HIV Protease Inhibitors
Myoglobin
Nitric Oxide Synthase
Peroxidase
Tuberculosis



Chapters, Reviews, Books, and Patents


Primary Papers (selected)

2008

Efficient Catalytic Turnover of Cytochrome P450cam is Supported by a T252N Mutation. Kim, D., Heo, Y.-S., Ortiz de Montellano, P.R. (2008) Arch. Biochem. Biophys. In press.

Selenolate Complexes of CYP101 and the Heme-bound hHO-1/H25A Proximal Cavity Mutant. Jiang, Y., and Ortiz de Montellano, P.R. (2008) Inorg. Chem. In press.

Mycobacterium tuberculosis CYP130: Crystal Structure, Biophysical Characterization, and Interactions with Antifungal Azole Drugs. Ouellet, H., Podust, L. M., and Ortiz de Montellano, P. R. (2008) J. Biol. Chem. 283, 5069-5080.

Crystal Structure and Properties of CYP231A2 from the Thermoacidophilic Archaeon Picrophilus torridus. Ho, W. W., Li, H., Nishida, C. R., Ortiz de Montellano, P. R., and Poulos, T. L. (2008) Biochemistry 47, 2071-2079.

Shielding of Peroxidase Heme Vinyl Groups from Autocatalytically Generated Electrophilic Metabolites. Wojciechowski, G., and Ortiz de Montellano, P. R. (2008) Acta Chim. Slov. 55, 75-84.

Implication for Using Heme Methyl Contact Shifts as Indicators of Heme Seating as Related to Stereoselectivity in the Catabolism of Heme by Heme Oxygenase: In-plane Heme Versus Axial His Rotation. Ogura, H., Evans, J. P., Ortiz de Montellano, P. R., and LaMar, G. N. (2008) Biochemistry, 47, 421-430.

2007

Inhibition of the Mycobacterium tuberculosis Enoyl Acyl Carrier Protein Reductase InhA by Arylamides. He, X., Alian, A., and Ortiz de Montellano, P. R. (2007) Bioorg. Med. Chem. 15, 6649-6658.

Interdomain Interactions Within the Two-Component Heme-Based Sensor Protein DevS from Mycobacterium tuberculosis. Yukl, E.T., Ioanoviciu, A., Ortiz de Montellano, P.R., and Moënne-Loccoz, P. (2007) Biochemistry, 46, 9728-9736.

Functional Expression and Characterization of Cytochrome P450 52A21 from Candida albicans. Kim, D., Cryle, M.J., De Voss, J.J., and Ortiz de Montellano, P.R. (2007) Arch. Biochem. Biophys. 464, 213-220.

Arthromyces ramosus Peroxidase Produces Two Chlorinating Species. Huang, L., and Ortiz de Montellano, P. R. (2007) Biochem. Biophys. Res. Commun. 355, 581-586.

DevS, a Heme-Containing Two-Component Oxygen Sensor of Mycobacterium tuberculosis. Ioanoviciu, A., Yukl, E. T., Moënne-Loccoz, P., Ortiz de Montellano, P. R. (2007) Biochemistry 46, 4250-4260.

Radical Energies and the Regiochemistry of Addition to Heme Groups. Methylperoxy and Nitrite Radical Additions to Heme of Horseradish Peroxidase. Wojciechowski, G., and Ortiz de Montellano, P. R. (2007) J. Am. Chem. Soc. 129, 1663-1672.

Motexafin Gadolinium-Induced Cell Death Correlates with HO1 Expression and Inhibition of P450 Reductase-Dependent Activities. Evans, J. P., Xu, F., Sirisawad, M., Miller, R., Naumovski, L., and Ortiz de Montellano, P. R. (2007) Mol. Pharm. 71, 193-200.

Mechanism of O2 Activation by Cytochrome P450cam Studied by Isotope Effects and Transient State Kinetics. Purdy, M. M., Koo, L.S., Ortiz de Montellano, P. R., Klinman, J. P. (2007) Biochemistry 45, 15793 - 15806.

Fungal Heme Oxygenases: Functional Expression and Characterization of Hmx1 from Saccharomyces cerevisiae and CaHmx1 from Candida albicans. Kim, D., Yukl, E.T., Moënne-Loccoz, P., and Ortiz de Montellano, P. R. (2007) Biochemistry 45, 14772-14780.

2006

The Diagnostic Substrate Bicyclohexane Reveals a Radical Mechanism for Bacterial Cytochrome P450 in Whole Cells. Austin, R. N., Deng, D., Jiang, Y., Luddy, K., van Beilen, J., B. Ortiz de Montellano, P. R., and Groves, J. T. (2006) Angew. Chem. Int. Ed. 45, 8192-8194.

Cytochrome P450 Active Site Plasticity. Attenuation of Imidazole Binding in Cytochrome P450cam by an L244A Mutation. Verras, A., Alian, A., and Ortiz de Montellano, P. R. (2006) Protein Eng. Design Selection 19, 491-496.

Pyrrolidine Carboxamides as a Novel Class of Inhibitors of Enoyl Acyl Carrier Protein Reductase of Mycobacterium tuberculosis. He, X., Alian, A., Stroud, R., and Ortiz de Montellano, P. R. (2006) J. Med. Chem. 49, 6308-6323.

CYP4 Isoform specificity in the -Hydroxylation of Phytanic Acid, a Potential Route to Elimination of the Causative Agent of Refsum's Disease. Xu, F., Ng, V. Y., Kroetz, D. L., and Ortiz de Montellano, P. R. (2006) J. Pharm. Exp. Therap. 318, 835-839.

Role of Heme-Protein Covalent Bonds in Mammalian Peroxidases. Protection of the Heme by a Single Engineered Heme-Protein Link in Horseradish Peroxidase. Huang, L., Wojciechowski, G., and Ortiz de Montellano, P. R. (2006) J. Biol. Chem. 281, 18983-18988.

Oxidative Activation of Thiacetazone by the Mycobacterium tuberculosis Flavin Monooxygenase EtaA and Human FMO1 and FMO3. Qian, L., and Ortiz de Montellano, P. R. (2006) Chem. Res, Toxicol. 19, 443-449.

Heme-Protein Covalent Bonds in Peroxidases and Resistance to Heme Modification During Halide Oxidation. Huang, L., and Ortiz de Montellano, P. R. (2006) Arch. Biochem. Biophys. 446, 77-83.

Radical Intermediates in the Catalytic Oxidation of Hydrocarbons by Bacterial and Human Cytochrome P450 Enzymes. Jiang, Y., He, X., and Ortiz de Montellano, P. R. (2006) Biochemistry 45, 533-542.

Alteration of the Regiospecificity of Human Heme Oxygenase-1 by Unseating of the Heme but not Disruption of the Distal Hydrogen Bonding Network. Wang, J., Evans, J. P., Ogura, H., La Mar, G. N., and Ortiz de Montellano, P. R. (2006) Biochemistry, 45, 61-73.

Solution NMR Study of Environmental Effects of Substrate Seating in Human Heme Oxygenase: Influence of Polypeptide Truncation, Substrate Modification, and Axial Ligand. Zhu, W.Z., Li, Y., Wang, J., Ortiz de Montellano, P. R., and La Mar, G. (2006) J. Inorg. Biochem. 100, 97-107.

2005

The Role of the Met-Tyr-Trp Crosslink in Mycobacterium tuberculosis Catalase-Peroxidase (KatG) as Revealed by KatG(M255I). Ghiladi, R. A., Medzihradszky, K. F., and Ortiz de Montellano, P. R. (2005) Biochemistry 44, 15093-15105.

Correlation Betweeen Isoniazid Resistance and Superoxide Reactivity in Mycobacterium tuberculosis KatG. Ghiladi, R. A., Medzihradszky, K. F., Rusnak, F. M., and Ortiz de Montellano, P. R. (2005) J. Am. Chem. Soc. 127, 13428-13442.

Autocatalytic Modification of the Prosthetic Heme of Horseradish but not Lactoperoxidase by Thiocyanate Oxidation Products. A Role for Heme-Protein Covalent Crosslinking. Wojciechowski, G., Huang, L, and Ortiz de Montellano, P. R. (2005) J. Am. Chem. Soc., 127, 15871-15879.

Calibration of the Channel that Determines the -Hydroxylation Regiopecificity of Cytochrome P4504A1. Catalytic Oxidation of 12-Halododecanoic Acids. He, X., Cryle, M. J., De Voss, J. J., and Ortiz de Montellano, P. R. (2005) J. Biol. Chem. 280, 22697-22705.

The Met-Tyr-Trp Crosslink in Mycobacterium tuberculosis Catalase-Peroxidase (KatG): Autocatalytic Formation and Effect on Enzyme Catalysis and Spectroscopic Properties. Ghiladi, R.A., Knudsen, G.M., Medzihradszky, K.F., and Ortiz de Montellano, P.R. (2005) J. Biol. Chem. 280, 22651-22663.

Prosthetic Heme Modification during Halide Ion Oxidation. Demonstration of Chloride Oxidation by Horseradish Peroxidase. Huang, L., Wojciechowski, G., and Ortiz de Montellano, P. R. (2005) J. Am. Chem. Soc. 127, 5345-5353.

Cyclopropyl Containing Fatty Acids as Mechanistic Probes for Cytochrome P450. Cryle, M.J., Ortiz de Montellano, P.R., De Voss, J.J. (2005) J. Org. Chem. 70, 2455-2469.

Crystal Structures of the G139A, G139A-NO, and G143H Mutants of Human Heme Oxygenase-1. A Finely Tuned Hydrogen Bonding Network Controls Oxygenase versus Peroxidase Activity. Lad, L., Koshkin, A., Ortiz de Montellano, P. R., and Poulos, T. L. (2005) J. Biol. Inorg. Chem. 10, 138-146.

Identification of the Myoglobin Tyrosyl Radical by Immuno-Spin Trapping and its Dimerization. Detweiler, C. D., Lardinois, O. M., Deterding, L. J., Ortiz de Montellano, P. R., Tomer, K. B., and Mason, R. P. (2005) Free Rad., Biol. Med. 38, 969-976.

2004

The Sulfolobus sulfataricus Electron Donor Partners of Thermophilic CYP119. An Unusual Non-NAD(P)H-Dependent Cytochrome P450 System. Puchkaev, A. V., and Ortiz de Montellano, P. R. (2004) Arch. Biochem. Biophys. 434, 169-177.

The P450cam/G248E Mutant Covalently Binds Its Prosthetic Heme Group. Limburg, J., LeBrun, L.A., and Ortiz de Montellano. P. R. (2004) Biochemistry 44, 4091-4099.

Regiospecificity Determinants of Human Heme Oxygenase. Differential NADPH- and Ascorbate-Dependent Heme Cleavage by the R183E Mutant. Wang, J., Lad, L., Poulos, T.L., and Ortiz de Montellano, P.R. (2005) J. Biol. Chem. 280, 2797-2806.

Protein Kinase Akt/PKB Phosphorylates Heme Oxygenase-1 in Vitro and in Vivo. Salinas, M., Wang, J., de Sagarra, M. R., Martin, D., Rojo, A. I., Martin-Perez, J., Ortiz de Montellano, P. R., and Cuadrado, A. (2004) FEBS Letters, 578, 90-94.

Radical Rebound Mechanism in Cytochrome P450-Catalyzed Hydroxylation of the Multifaceted Radical Clocks alpha- and beta-Thujone. He, X., and Ortiz de Montellano, P. R. (2004) J. Biol. Chem. 279, 39479-39484.

Oxidation of Carboxylic Acids by Horseradish Peroxidase Results in Prosthetic Heme Modification and Inactivation. Huang, L., Colas, C., and Ortiz de Montellano, P. R. (2004) J. Am. Chem. Soc. 126, 12865-12873.

Human Heme Oxygenase Oxidation of alpha- and gamma-meso-Phenylhemes. Wang, J., Niemevz, F., Lad, L., Huang, L., Alvarez, D. E., Buldain, G., Poulos, T. L., and Ortiz de Montellano, P. R. (2004) J. Biol. Chem. 279, 42593-42604.

EpoK, a Cytochrome P450 Involved in Biosynthesis of the Anticancer Agents Epothilones A and B. Substrate-Mediated Rescue of a P450 Enzyme. Ogura. H., Nishida, C. R., Hoch, U. R., Perera, R., Dawson, J. H., and Ortiz de Montellano, P. R. (2004) Biochemistry 43, 14712-14721.

alpha- and beta-Thujone Radical Rearrangements and Isomerizations. A New Radical Clock. He, X., and Ortiz de Montellano, P. R. (2004) J. Org. Chem. 69, 5684-5789.

Crystal Structures of Ferrous and Ferrous-NO Forms of Verdoheme in a Complex with Human Heme Oxygenase-1: Catalytic Implications for Heme Cleavage. Lad, L., Ortiz de Montellano, P. R., and Poulos, T. L. (2004) J. Inorg. Biochem., 98, 1686-1695.

Methyl Groups as Probes for Proteins and Complexes in In-Cell NMR Experiments. Serber, Z., Straub, W., Corsini, L., Nomura, A. M., Shimba, N., Craik, C. S., Ortiz de Montellano, P. R., and Dötsch, V. (2004) J. Am. Chem. Soc. 126, 7119-7125.

Intermolecular Interactions in the AhpC/AhpD Antioxidant Defense System of Mycobacterium tuberculosis. Koshkin, A., Knudsen, G. M., and Ortiz de Montellano, P. R. (2004) Arch. Biochem. Biophys. 427, 41-47.

Horseradish Peroxidase Mutants that Autocatalytically Modify their Prosthetic Heme Group. Insights into Mammalian Peroxidase Heme-Protein Covalent Bonds. Colas, C., and Ortiz de Montellano, P. R. (2004) J. Biol. Chem. 279, 24131-24140.

Inhibition of Mycobacterium tuberculosis AhpD, an Element of the Peroxiredoxin Defense Against Oxidative Stress. Koshkin, A., Zhou, X., Kraus, C. N., Brenner, J. M., Bandyopadhyay, P., Kuntz, I. D., Barry, C. E., III, and Ortiz de Montellano, P. R. (2004) Antimicrob. Agents Chemother. 48, 2424-2430.

Computer-Assisted Design of Selective Imidazole Inhibitors for Cytochrome P450 Enzymes. Verras, A., Kuntz, I. D., and Ortiz de Montellano, P. R. (2004) J. Med. Chem. 47, 3572-3579.

Autoreduction of Ferryl Myoglobin: Discrimination Among the Three Tyrosines and Two Tryptophan Residues as Electron Donors. Lardinois, O. M., and Ortiz de Montellano, P. R. (2004) Biochemistry 43, 4601-4610.

Cyclopropyl Fatty Acids Implicate a Radical but not a Cation as an Intermediate in P450BM3-Catalyzed Hydroxylations. Cryle, M. J., Stuthe, J.M.U., Ortiz de Montellano, P. R., and De Voss, J. J. (2004) Chem. Commun. 5, 512-513.

Superoxide Reactivity of KatG: Insights into the Isoniazid Resistance Pathways in TB. Ghiladi, R. A., Cabelli, D. E., and Ortiz de Montellano, P. R. (2004) J. Am. Chem. Soc. 126, 4772-4773.

Catalytic Activity and Isoform-Specific Inhibition of Rat Cytochrome P450 4F Enzymes. Xu, F., Falck, J. R., Ortiz de Montellano, P. R., and Kroetz, D. L. (2004) J. Pharmacol. Exp. Therap. 308, 887-895.

Crystal Structure of Human Heme Oxygenase-1 in a Complex with Biliverdin. Lad, L., Friedman, J., Li, H., Bhaskar,B., Ortiz de Montellano, P. R., and Poulos, T. L. (2004) Biochemistry 43, 3793-3801.

Communication Between the Active Site and Dimer Interface of a Herpesvirus Protease Revealed by a Transition-state Inhibitor. Marnett, A. B., Nomura, A. M., Shimba, N., Ortiz de Montellano, P. R., and Craik, C. S. (2004) Proc. Natl. Acad. Sci. U.S.A. 101, 6870-6875.

1H NMR Investigation of the Solution Structure of Substrate-free Human Heme Oxygenase: Comparison to the Cyanide-inhibited, Substrate-bound Complex. Li, Y., Syvitski, R. T., Auclair, K., Ortiz de Montellano, P. R., and La Mar, G. N. (2004) J. Biol. Chem. 279, 10195-10205.

Steady State Kinetic Investigation of Cytochrome P450cam: Interaction with Redox Partners and Reaction with Molecular Oxygen. Purdy, M. M., Koo, L. S., Ortiz de Montellano, P. R., and Klinman, J. P. (2004) Biochemistry 43, 271-281.

2003

Intra- and Intermolecular Transfers of Protein Radicals in the Reactions of Sperm Whale Myoglobin with Hydrogen Peroxide. Lardinois, O. M., and Ortiz de Montellano, P. R. (2003) J. Biol. Chem. 278, 36214-36226.

Crystal Structures of Epothilone D-bound, Epothilone-B bound, and Substrate-free Forms of Cytochrome P450epoK. Nagano, S., Li, H., Shimizu, H., Nishida, C R., Ogura, H., Ortiz de Montellano, P. R., and Poulos, T. L. (2003) J. Biol. Chem. 278, 44886-44893.

Solution 1H,15N NMR Spectroscopic Characterization of Substrate-Bound Cyanide-Inhibited, Human Heme Oxygenase: Water Occupation of the Distal Cavity. Li, Y., Syvitski, R. T., Auclair, K., Ortiz de Montellano, P. R., and La Mar, G. N. (2003) J. Am. Chem. Soc. 125, 13392-13403.

Crystal Structures of the Ferric, Ferrous, and Ferrous-NO forms of the Asp140Ala Mutant of Human Heme Oxygenase-1: Catalytic Implications. Lad, L., Wang, J., Li, H., Friedman, J., Bhaskar, B., Ortiz de Montellano, P. R., and Poulos, T. L. (2003) J. Mol. Biol. 330, 527-538.

Nitric-oxide Synthase (NOS) Reductase Domain Models Suggest a New Control Element in Endothelial NOS that Attenuates Calmodulin-dependent Activity. Knudsen, G. M., Nishida, C. R., Mooney, S. D., and Ortiz de Montellano, P. R. (2003) J. Biol. Chem. 278, 31814-31824.

he Mechanism of Mycobacterium tuberculosis Alkylhydroperoxidase AhpD as Defined by Mutagenesis, Crystallography, and Kinetics. Koshkin, A., Nunn, C. M., Djordjevic, S., and Ortiz de Montellano, P. R. (2003) J. Biol. Chem. 278, 29502-29508.

The Binding Sites on Human Heme Oxygenase-1 for Cytochrome P450 Reductase and Biliverdin Reductase. Wang, J., and Ortiz de Montellano, P. R. (2003) J. Biol. Chem. 278, 20069-10076.

Tryptophan-14 is the Preferred Site of DBNBS Spin Trapping in the Self-Peroxidation Reaction of Sperm Whale Metmyoglobin with a Single Equivalent of Hydrogen Peroxide. Gunther, M. R., Tschirret-Guth, R. A., Lardinois, O. M., and Ortiz de Montellano, P. R. (2003) Chem. Res. Toxicol. 16, 652-660.

Comparison of the Heme-Free and -Bound Crystal Structures of Human Heme Oxygenase-1. Lad, L., Schuller, D. J., Shimizu, H., Friedman, J., Li, H., Ortiz de Montellano, P. R., and Poulos, T. L. (2003) J. Biol. Chem. 278, 7834-7843.

Cloning and Expression of a Heme Binding Protein from the Genome of Saccharomyces cerevisia. Auclair, K., Huang,H.-W., Moënne-Loccoz, P., and Ortiz de Montellano, P. R. (2003) Protein Express. Purific. 28, 340-349.

Interaction of Nitric Oxide with Human Heme Oxygenase-1. Wang, J., Lu, S., Moënne-Loccoz, P., and Ortiz de Montellano, P. R. (2003) J. Biol. Chem. 278, 2341-2347.

Aromatic Stacking as a Determinant of the Thermal Stability of CYP119 from Sulfolobus solfataricus. Puchkaev, A. V., Koo, L. S. K., and Ortiz de Montellano, P. R. (2003) Arch. Biochem. Biophys. 409, 52-58.

2002

Cytochrome P450 3A4-Mediated Oxidative Conversion of a Cyano to an Amide Group in the Metabolism of Pinacidil. Zhang, Z., Li, Y., Stearns, R. A., Ortiz de Montellano, P. R., Baillie, T. A., and Tang, W. (2002) Biochemistry, 41, 2712-2718.  HTML, PDF

Asp225 and Glu375 in Autocatalytic Attachment of the Prosthetic Heme Group of Lactoperoxidase. Colas, C., Kuo, J. M., and Ortiz de Montellano, P. R. (2002) J. Biol. Chem., 277, 7191-7200. HTML, PDF

The Antituberculosis Drug Ethionamide is Activated by a Flavoprotein Monoxygenase. Vannelli, T., Dykman, A., and Ortiz de Montellano, P. R. (2002) J. Biol. Chem., 277, 12824-12829. HTML, PDF

Autocatalytic Mechanism and Consequences of Covalent Heme Attachment in the Cytochrome P4504A Family. Lebrun, L. A., Hoch, U., and Ortiz de Montellano, P. R. (2002) J. Biol. Chem., 277, 12755-12761. HTML, PDF

Covalent Attachment of the Heme Prosthetic Group in the CYP4F Cytochrome P450 Family. LeBrun, L. A., Xu, F., Kroetz, D. L., and Ortiz de Montellano, P. R. (2002) Biochemistry , 41, 5931-5937. HTML, PDF

Revisiting the Mechanism of P450 Enzymes Using the Radical Clocks Norcarane and Spiro[2,5]bicyclooctane. Auclair, K., Hu, Z., Little, D. M., Ortiz de Montellano, P. R., and Groves, J. T. (2002) J. Am. Chem. Soc., 124, 6020-6027. HTML, PDF

The Crystal Structure of Mycobacterium tuberculosis Alkylhydroperoxidase AphD, a Potential Target for Antitubercular Drug Development. Nunn, C. M., Djordjevic, S., Hillas, P. J., Nishida, C. R., and Ortiz de Montellano, P. R. (2002) J. Biol. Chem., 277, 20033-20040. HTML, PDF

Ehanced Electron Transfer and Lauric Acid Hydroxylation by Site-Directed Mutagenesis of CYP199. Koo, L. S., Immoos, C. E., Cohen, M. S., Farmer, P. J., and Ortiz de Montellano, P. R. (2002) J. Am. Chem. Soc., 124, 5684-5691. HTML, PDF

Cytochrome P450cin (CYP176A): Isolation, Expression, and Characterization. Hawkes, D. B., Adams, G., Burlingame, A., Ortiz de Montellano, P. R., and De Voss, J. J. (2002) J. Biol. Chem., 277, 27725-27732. HTML, PDF

Antihypertensive Effect of Mechanism-Based Inhibition of Renal Arachidonic Acid w-Hydroxylase Activity. Xu, F., Pak, W., Su, P., Straub, W. O., Ortiz de Montellano, P. R., and Kroetz, D. L. (2002) Am. J. Physiol.: Regulatory, Integrative and Comparative Physiology. in press.

Influence of the Distal His in Imparting Imidazolate Character to the Proximal His in Heme Peroxidase: 1H NMR Study of Cyanide-Inhibited His42Ala Horseradish Peroxidase. De Ropp, J. S., Sham, S., Asokan, A., Newmyer, S., Ortiz de Montellano, P. R., and La Mar, G. N. (2002) J. Am. Chem. Soc. in press

Solution NMR Characterization of an Unusual Distal H-Bond Network in the Active Site of the Cyanide-Inhibited Human Heme Oxygenase Complex of the Symmetric Substrate 2,4-Dimethyldeuterohemin. Li, Y., Syvitiski, R., Auclair, K., Wilks, A., Ortiz de Montellano, P. R., and La Mar, G. N. (2002) J. Biol. Chem., in press.

Aromatic Stacking as a Determinant of the Thermal Stability of CYP119 from Sulfolobus solfataricus. Puchkaev, A. V., Koo, L. S. K., and Ortiz de Montellano, P. R. (2002) Arch. Biochem. Biophys. in press.

Tryptophan-14 is the Preferred Site of DBNBS Spin Trapping in the Self-Peroxidation Reaction of Sperm Whale Metmyoglobin. Gunther, M. R., Tschirret-Guth, R. A., Lardinois, O. M., and Ortiz de Montellano, P. R. (2002) Submitted for publication.

1H NMR Detection of Immobilized Water Molecules within a Strong Hydrogen-Bonding Distal Network in Substrate-bound Human Heme Oxygenase. Syvitski, R. T., Li, Y., Auclair, K., Ortiz de Montellano, P. R., and La Mar, G. N. (2002) Submitted for publication.

The Genome of Saccharomyces cerevisiae does not Include a Heme Oxygenase. Cloning and Expression of a Novel Heme Protein. Auclair, K., Moënne-Loccoz, P., and Ortiz de Montellano, P. R. (2002) Submitted for publication.

2001

Disruption of an Active Site Hydrogen bond converts Human Heme Oxygenase-1 into a Peroxidase. Lightning, L.K., Huang, H., Moenne-Loccoz, P., Loehr, T.M., Schuller, D.J., Poulos, T.L., and Ortiz de Montellano, P.R. (2001) J. Biol. Chem. 276, 10612-10619. HTML,  PDF

Reversible Pressure Deformation of a Thermophilic Cytochrome P450 Enzyme (CYP119) and its Active-Site Mutants. Tschirret-Guth, R.A., Koo, L.S., Hui Bon Hoa, G., and Ortiz de Montellano, P.R. (2001) J. Am. Chem. Soc. 123, 3412-3417. HTML,  PDF

Covalently-linked Heme in Cytochrome P4504A Fatty Acid Hydroxylases. Hoch, U., and Ortiz de Montellano, P.R. (2001) J. Biol. Chem. 276, 11339-11346. HTML,  PDF

Roles of the Proximal Heme Thiolate Ligand of Cytochrome P450cam. Auclair, K., Moenne-Loccoz, P., and Ortiz de Montellano, P.R. (2001) J. Am. Chem. Soc. 123, 4877-4885. HTML,  PDF

Solution 1H NMR of the Molecular and Electronic Structure of the Heme Cavity and Substrate Binding Pocket of High-Spin Ferric Horseradish Peroxidase: Effect of His42Ala Mutation. Asokan, A., de Ropp, J.S., Newmyer, S.L., Ortiz de Montellano, P.R., and La Mar, G.N. (2001) J. Am. Chem. Soc. 123, 4243-4254. HTML,  PDF

Solution 1H NMR of the Active Site of Substrate-Bound, Cyanide-Inhibited, Human Heme Oxygenase: Comparison to the Crystal Structure of the Water-ligated Form. La Mar, G. N., Asokan, A., Espiritu, B., Yeh, D. C., Auclair, K., and Ortiz de Montellano, P. R. (2001) J. Biol. Chem. 276, 15676-15687. HTML,  PDF

Control of Electron Transfer in Nitric Oxide Synthases. Swapping of Autoinhibitory Elements among Nitric Oxide Synthase Isoforms. Nishida, C. R., and Ortiz de Montellano, P. R. (2001) J. Biol. Chem. 267, 20116-20124. HTML,  PDF

H2O2-Mediated Cross-Linking between Lactoperoxidase and Myoglobin. Elucidation of Protein -Protein Radical Transfer Reactions. Lardinois, O. M., and Ortiz de Montellano, P. R. (2001) J. Biol. Chem., J. Biol. Chem. 276, 23186-23191. HTML,  PDF

Identification of Novel Cellular Proteins that Bind to the LC8 Dynein Light Chain Using a Pepscan Technique. Rodriguez-Crespo, I., Yélamos, B., Roncal, F., Albar, J. P., Ortiz de Montellano, P. R., and Gavilanes, F. (2001) FE BS Lett. 503, 135-141. H TML,  PDF

2000

Structural Determination of the Substrate Specificities and Regioselectivities of the Rat and Human Fatty Acid w -Hydorxylases. Hoch, U., Zhang, Z., Kroetz, D.L., and Ortiz de Montellano, P.R. (2000) Arch. Biochem. Biophys. 373, 63-71. Abstra ct, PD F

Omega-Hydroxyceramides are Required for Covalently-Bound Lipid Envelope (CLE) Formation and Normal Epidermal Permeability Barrier Function. Behne, M., Uchida, Y., Seki, T., Ortiz de Montellano, P.R., Elias, P.M., and Holleran, W.M. (2000) J. Invest. Dermatol. 114, 185-192.  HTML, PDF

Reaction Intermediates and Single Turnover Rate Constants for the Oxidation of Heme by Human Heme Oxygenase-1. Liu, Y., and Ortiz de Montellano, P.R. (2000) J. Biol. Chem. 275, 5297-5307.  HTML, PDF

Protein Control of the Formation and Decomposition of CYP119 and CYP110 Aryl-Iron Complexes. Tschirret-Guth, R.A., Koo, L.S., and Ortiz de Montellano, P.R. (2000) J . Biol. Inorg. Chem. 5, 204-212. Abstract, PDF

The Active Site of the Thermophilic CYP119 from Sulfolobus solfataricus. Koo, L.S., Tschirret-Guth, R.A., Straub, W., Moenne-Loccoz, P., Loehr, T.M., and Ortiz de Montellano, P.R. (2000) J. Biol. Chem. 275, 14112-14123.  HTML, PDF

The AhpC and AhpD Antioxidant Defense System of Mycobacterium tuberculosis. Hillas, P.J., Soto del Alba, F., Oyarzabal, J., Wilks, A., and Ortiz de Montellano, P.R. (2000) J. Biol. Chem. 275, 18801-18809.  HTML, PDF

EPR Spin-Trapping of a Myeloperoxidase Protein Radical. Lardinois, O.M., and Ortiz de Montellano, P.R. (2000) Biochem. Biophys. Res. Commun. 270, 199-202.  Abstra ct, PD F

Rational Design of Selective Submicromolar Inhibitors of Tritrichomonas foetus Hypoxanthine-Guanine-Xanthine Phosphoribosyltransferase. Aronov, A.M., Munagala, N.R., Ortiz de Montellano, P.R., Kuntz, I.D., and Wang, C.C. (2000) Biochemistry . 39, 4684-4691.  HTML, PDF

Molecular Basis for the w-Regiospecificity of the CYP4A2 and CYP4A3 Fatty Acid Hydroxylases. Hoch, U., Falck, J.R., and Ortiz de Montellano, P.R. (2000) J. Biol. Chem. 275, 26952-26958.  HTML, PDF

Crystal Structure of a Thermophilic Cytochrome P450 from the Archaeon Sulfolobus solfataricus. Yano, J.K., Koo, L.S., Schuller, D.J., Li, H., Ortiz de Montellano, P.R., and Poulos, T.L. (2000) J. Biol. Chem. 275, 31086-31092.  HTML, PDF

Replacement of the Distal Glycine-139 Transforms Human Heme Oxygenase-1 into a Peroxidase. Liu, Y., Lightning, L.K., Huang, H.-W., Moenne-Loccoz, P., Schuller, D.J. Loehr, T.M., Poulos, T.L., and Ortiz de Montellano, P.R. (2000) J. Biol. Chem. 275, 34501-34507.  HTML, PDF

An Ala245Thr Mutation Conveys on Cytochrome P450eryF the Ability to Oxidize Alternative Substrates. Xiang, H., Tschirret-Guth, R.A., and Ortiz de Montellano, P.R. (2000) J. Biol. Chem. 275, 35999-36006.  HTML, PDF

Reactivities of Organic Phase Biosensors 3: Electrochemical Study of Cytochrome P450cam Immobilised in a Methyltriethoxysilane Sol-Gel. Iwuoha, E.I., Kane, S., Ania, C.O., Smyth, M.R., Ortiz de Montellano, P.R., and Fuhr, U. (2000) Electroana lys is. 12, 980-986.  Abstract, PDF

1999

AMP-Activated Protein Kinase Phosphorylation of Endothelial NO Synthase. Chen, Z.-P., Mitchelhill, K. I., Michell, B. J., Stapleton, D. Rodríguez-Crespo, I., Witters, L. A., Power, D. A., Ortiz de Montellano, P. R., and Kemp, B. E. (1999) FEBS Letters 443, 285-289.

Reduction of Peroxides and Dinitrobenzenes by Mycobacterium tuberculosis Thioredoxin and Thioredoxin Reductase. Zhang, Z., Hillas, P. J., and Ortiz de Montellano, P. R. (1999) Arch. Biochem. Biophys. 363, 19-26. HTML  PDF

Nitric Oxide Synthases Catalyze the Activation of Redox Cycling and Bioreductive Anticancer Agents. Garner, A. P., Paine, M. J. I., Rodríguez-Crespo, I., Chinje, E. C., Ortiz de Montellano, P. R., Stratford, I. J., Tew, D. G., and Wolf, C. R. (1999) Cancer Research 59, 1929-1934.

Replacement of the Proximal Histidine Iron Ligand by a Cysteine or Tyrosine Converts Heme Oxygenase to an Oxidase. Liu, Y., Moënne-Loccoz, P., Hildebrand, D., Wilks, A., Loehr, T. M., Mauk, A. G., and Ortiz de Montellano, P. R. (1999) Biochemistry 38, 3733-3743. HTML  PDF

Autoinhibition of Endothelial Nitric Oxide Synthase. Identification of an Electron Transfer Control Element. Nishida, C. R. and Ortiz de Montellano, P. R. (1999) J. Biol. Chem. 274, 14692-14698. HTML, PDF 

Trifluoromethyldiazirinylphenyldiazenes: New Hemoprotein Active Site Probes. Tschirret-Guth, R. A., Medzihradszky, K. F., and Ortiz de Montellano, P. R. (1999) J. Am. Chem. Soc. 121, 4731-4737. HTML, PDF 

Mutation of the Five Conserved Histidines in the Endothelial Nitric Oxide Synthase Hemoprotein Domain. Rodríguez-Crespo, I., Nishida, C. R., Knudsen, G. M., and Ortiz de Montellano, P. R. (1999) J. Biol. Chem. 274, 21617-21624. HTML, PDF

Assignment of the Heme Axial Ligand(s) for the Ferric Myoglobin (H93G) and Heme Oxygenase (H25A) Cavity Mutants as Oxygen Donors Using Magnetic Circular Dichroism. Pond, A. E., Roach, M. P., Sono, M., Rux A. H., Franzen, S., Hu, R., Ramser-Thomas, M., Wilks, A., Ikeda-Saito, M., Ortiz de Montellano, P. R., Woodruff, W. H., Boxer, S. G., and Dawson, J. H. (1999) Biochemistry  38, 7601-7608.  HTML

Deuterium Magic Angle Spinning Studies of Substrates Bound to Cytochrome P450.  Lee, H., Ortiz de Montellano, P.R., and McDermott, A.E. (1999) Biochemistry  38, 10808-10813.  HTML

Crystal Structure of Human Heme Oxygenase-1. Schuller, D., Wilks, A., Ortiz de Montellano, P.R., and Poulos, T. (1999) Nature Struct. Biol. 6, 860-867.

The Akt Kinase Signals Directly to Endothelial Nitric Oxide Synthase. Michell, B.J., Griffiths, J.E., Mitchelhill, K.I., Rodriguez-Crespo, I., Tiganis, T., Bozinovski, S., Ortiz de Montellano, P.R., Kemp, B.E., and Pearson, R.B. (1999) Current Biol. 9, 845-848. Abrstract

Spin-Trapping and Protein-Crosslinking of the Lactoperoxidase Protein Radical. Lardinois, O.M., Medzihradszky, K.F., and Ortiz de Montellano, P.R. (1999) J. Biol. Chem. 274, 35441-35448.  HTML, PDF

1998

Electron Transfer and Catalytic Activity of Nitric Oxide Synthases. Chimeric Constructs of the Neuronal, Inducible, and Endothelial Isoforms. Nishida, C. R. and Ortiz de Montellano, P. R. (1998) J. Biol. Chem. 273, 5566-5571. HTML PDF

Site-Specific Spin Trapping of Tyrosine Radicals in the Oxidation of Metmyoglobin by Hydrogen Peroxide. Gunther, M. R., Tschirret-Guth, R. A., Witkowska, H. E., Fann, Y. C., Barr, D. P., Ortiz de Montellano, P. R., and Mason, R. P. (1998) Biochem. J. 330, 1293-1299. HTML PDF

Glu-320 and Asp-323 are Determinants of the CYP4A1 Hydroxylation Regiospecificity and Resistance to Inactivation by 1-Aminobenzotriazole. Dierks, E. A., Davis, S. C., and Ortiz de Montellano, P. R. (1998) Biochemistry 37, 1839-1847. HTML  PDF

Horseradish Peroxidase: Partial Rescue of the His-42 -> Ala Mutant by a Concurrent Asn-70 -> Asp Mutation. Savenkova, M. I. and Ortiz de Montellano, P. R. (1998) Arch. Biochem. Biophys. 351, 286-293. HTML PDF

Heme Oxygenase Active-Site Residues Identified by Heme-Protein Cross-linking during Reduction of CBrCl3. Wilks, A., Medzihradsky, K., and Ortiz de Montellano, P. R. (1998) Biochemistry 37, 2889-2896. HTML PDF

Pressure-Induced Deformations of the Cytochrome P450cam Active Site. Tschirret-Guth, R., Ortiz de Montellano, P. R., and Hui Bon Hoa, G. (1998) J. Am. Chem. Soc. 120, 3590-3596. HTML  PDF

Drug Metabolism Biosensors: Electrochemical Reactivities of Cytochrome P450cam Immobilized in Synthetic Vesicular Systems. Iwuoha, E., Joseph, S., Zhang, Z., Smyth, M. R., Fuhr, U., and Ortiz de Montellano, P. R. (1998) J. Pharm. Biomed. Analysis 17 (6-7), 1101-1110.

Cytochrome P450cam Substrate Specificity: Relationship Between Structure and Catalytic Oxidation of Alkylbenzenes. Sibbesen, O., Zhang, Z., and Ortiz de Montellano, P. R. (1998) Arch. Biochem. Biophys. 353, 285-296. Abstract PDF

Improvement of Peroxygenase Activity by Reloaction of a Catalytic Histidine within the Active Site of Horseradish Peroxidase. Savenkova, M., Kuo, J. M., and Ortiz de Montellano, P. R. (1998) Biochemistry  37, 10828-10836. HTML PDF

The Substrate Specificity of Cytochrome P450cam . Zhang, Z., Sibbesen, O., Johnson, R. A., and Ortiz de Montellano, P. R. (1998) Bioorg. Med. Chem.  6, 1501-1508.  HTML, PDF

Specific Azidophenyldiazene Hemoprotein Active Site Probes. Cross-linking of the Heme to His-64 in Myoglobin. Tschirret-Guth, R. A., Medzihradszky, K. F., and Ortiz de Montellano, P. R. (1998) J. Am. Chem. Soc. 120, 7404-7410. HTML PDF

Neuronal Nitric Oxide Synthase Isoforms a and m Are Closely Related, Calpain-Sensitive Proteins. Lainé, R. and Ortiz de Montellano, P. R. (1998) Molec. Pharmacol. 54, 305-312. HTML PDF

Crystallization of Recombinant Human Heme Oxygenase-1. Schuller, D., Wilks, A., Ortiz de Montellano, P. R., and Poulos, T. L. (1998) Protein Science 7, 1836-1838.  Abstra ct

The Catalytic Site of Cytochrome P4504A11 (CYP4A11) and Its L131F Mutant. Dierks, E. A., Zhang, Z., Johnson, E. F., and Ortiz de Montellano, P. R. (1998) J. Biol. Chem. 273, 23055-23061. HTML  PDF

Solution 1H NMR Investigation of the Molecular and Electronic Structure of the Active Site of Substrate-Bound Human Heme Oxygenase: the Nature of the Distal Hydrogen Bond Donor to Bound Ligands. Gorst, C. M., Wilks, A., Yeh, D. C., Ortiz de Montellano, P. R., and La Mar, G. N. (1998) J. Am. Chem. Soc. 120, 8875-8884. HTML PDF

Binding of Dynein Light Chain (PIN) to Neuronal Nitric Oxide Synthase in the Absence of Inhibition. Rodríguez-Crespo, I., Straub, W., Gavilanes, F., and Ortiz de Montellano, P. R. (1998) Arch. Biochem. Biophys. 359, 297-304. HTML  PDF

Synthesis of Photoaffinity Probes for Heme-Containing Proteins. Tschirret-Guth, R. A. and Ortiz de Montellano, P. R. (1998) J. Org. Chem. 63, 9711-9715. PDF

1997

Peroxidation of a Specific Tryptophan of Metmyoglobin by Hydrogen Peroxide. DeGray, J. A., Gunther, M. R., Tschirret-Guth, R., Ortiz de Montellano, P. R., and Mason, R. P. (1997) J. Biol. Chem. 272, 2359-2362. HTML PDF

Active Site Topologies and Cofactor-Mediated Conformational Changes of Nitric Oxide Synthases. Gerber, N. C., Rodríguez-Crespo, I., Nishida, C. R., and Ortiz de Montellano, P. R. (1997) J. Biol. Chem. 272, 6285-6290. HTML PDF

Heme Oxygenase-1, Intermediates in Verdoheme Formation and the Requirement for Reduction Equivalents. Liu, Y., Moënne-Loccoz, P., Loehr, T., and Ortiz de Montellano, P. R. (1997) J. Biol. Chem. 272, 6909-6917. HTML PDF

Autocatalytic Processing of Heme by Lactoperoxidase Produces the Native Protein-Bound Prosthetic Group. DePillis, G. D., Ozaki, S., Kuo, J. M., Maltby, D. A., and Ortiz de Montellano, P. R. (1997) J. Biol. Chem. 272, 8857-8860. HTML PDF

Inactivation of the Inducible Nitric Oxide Synthase by Peroxynitrite. Hühmer, A. F. R., Nishida, C. R., Ortiz de Montellano, P. R., and Schöneich, C. (1997) Chem. Res. Toxicol. 10, 618-626. HTML  PDF

Substrate Docking Algorithms and Prediction of the Substrate Specificity of Cytochrome P450cam and its L244A Mutant. De Voss, J. J., Sibbesen, O., Zhang, Z. P., and Ortiz de Montellano, P. R. (1997) J. Am. Chem. Soc. 119, 5489-5498.  HTML PDF

Characterization of Human Liver Inducible Nitric Oxide Synthase Expressed in Escherichia coli. Gerber, N. C., Nishida, C. R., and Ortiz de Montellano, P. R. (1997) Arch. Biochem. Biophys. 343, 249-253. Abstract PDF

Endothelial Nitric Oxide Synthase: Modulations of the Distal Heme Site Produced by Progressive N-Terminal Deletions.  Rodríguez-Crespo, I., Moënne-Loccoz, P., Loehr, T. M., and Ortiz de Montellano, P. R. (1997) Biochemistry 36, 8530-8538. HTML PDF

The Discovery, Characterization, and Crystallographically Determined Binding Mode of an FMOC Containing Inhibitor of the HIV-1 Protease. Rutenber, E. E., De Voss, J. J., Hoffman, L., Stroud, R. M., Lee, K. H., Alvarez, J. C., McPhee, F., Craik, C. S., and Ortiz de Montellano, P. R. (1997) Bioorg. Med. Chem. 5, 1311-1320.  Abstract, PDF

Alkylation of a Catalytic Aspartate Group of the SIV Protease by an Epoxide Inhibitor. Caldera, P., Yu, Z., Knegtel, R. M. A., McPhee, F., Burlingame, A., Craik, C. S., Kuntz, I. D., and Ortiz de Montellano, P. R. (1997) Bioorg. Med. Chem. 5, 2019-2027.  Abstract, PDF

Oxidation of a-Meso-Formylmesoheme by Heme Oxygenase. Electronic control of the reaction regiospecificity. Torpey, J. and Ortiz de Montellano, P. R. (1997) J. Biol. Chem. 272, 22008-14. HTML PDF

1996

Active Site Topology of Human Cytochrome P450 2E1. Mackman, R., Guo, Z., Guengerich, F. P., and Ortiz de Montellano, P. R. (1996) Chem. Res. Toxicol. 9, 223-226. HTML  PDF

Heme Oxygenase (HO-1): His-132 Stabilizes a Distal Water Ligand and Assists Catalysis. Wilks, A., Ortiz de Montellano, P. R., Sun, J., and Loehr, T. M. (1996) Biochemistry 35, 930-936. HTML PDF

Peroxynitrite Reduction of Calmodulin Stimulation of Neuronal Nitric Oxide Synthase. Hühmer, A. F. R., Gerber, N. C., Ortiz de Montellano, P. R., and Schöneich, C. (1996) Chem. Res. Toxicol. 9, 484-491. HTML PDF

Oxidation of w-Oxofatty Acids by Cytochrome P450BM-3 (CYP102). Davis, C., Sui, Z., Peterson, J. A., and Ortiz de Montellano, P. R. (1996) Arch. Biochem. Biophys. 328, 35-42. Abstract PDF

Endothelial Nitric Oxide Synthase: Expression in Escherichia coli, Spectroscopic Characterization, and Role of Tetrahydrobiopterin in Dimer Formation. Rodríguez-Crespo, I., Gerber, N. C., and Ortiz de Montellano, P. R. (1996) J. Biol. Chem. 271, 11462-11467. HTML PDF

Rescue of the Catalytic Activity of an H42A Mutant of Horseradish Peroxidase by Exogenous Imidazoles. Newmyer, S. L. and Ortiz de Montellano, P. R. (1996) J. Biol. Chem. 271, 14891-14896.  HTML PDF

Irreversible Inhibition of the HIV-1 Protease: Targeting Alkylating Agents to the Catalytic Aspartate Groups. Wah, Y. Y., Caldera, P., De Voss, J. J., McPhee, F., Burlingame, A., Craik, C. S., Kuntz, I. D., and Ortiz de Montellano, P. R. (1996) J. Am. Chem. Soc. 118, 5846-5856.  HTML  PDF

Active Site Topologies of CYP2D6 and Its Aspartate-301 -> Glutamate and Asparagine Mutants. Mackman, R., Tschirret-Guth, R. A., Smith, G., Hayhurst, G. P., Ellis, S. W., Lennard, M. S., Tucker, G. T., Wolf, C. R., and Ortiz de Montellano, P. R. (1996) Arch. Biochem. Biophys. 331, 134-140. Abstract PDF

Ligation of the Iron in Heme Oxygenase: EXAFS, Electronic Absorption, and Magnetic Circular Dichroism Studies. Hawkins, B. K., Wilks, A., Dawson, J. H., Powers, L., and Ortiz de Montellano, P. R. (1996) Bio chim. Biophys. Acta 1295, 165-173.

Putidaredoxin Reductase-Putidaredoxin-Cytochrome P450cam Triple Fusion Protein. Construction of a Self-Sufficient Escherichia coli Catalytic System. Sibbesen, O., De Voss, J. J., and Ortiz de Montellano, P. R. (1996) J. Biol. Chem. 271, 22462-22469. HTML PDF

Oxidation of an a-meso-Methyl-Substituted Heme to an a-Biliverdin by Heme Oxygenase. A Novel Heme Cleavage Reaction. Torpey, J., Lee, D. A., Smith, K. M., and Ortiz de Montellano, P. R. (1996) J. Am. Chem. Soc. 118, 9172-9173. HTML PDF

Rescue of His-42 -> Ala Horseradish Peroxidase by a Phe-42 -> His Mutation. Engineering of a Surrogate Catalytic Histidine. Savenkova, M. I., Newmyer, S. L., and Ortiz de Montellano, P. R. (1996) J. Biol. Chem. 271, 24598-24603. HTML PDF

Oxidation of the Meso-Methylmesoheme Regioisomers by Heme Oxygenase.  Electronic Control of the Reaction Regiospecificity. Torpey, J. and Ortiz de Montellano, P. R. (1996) J. Biol. Chem. 271, 26067-26073. HTML PDF

Rescue of the Horseradish Peroxidase His-170 -> Ala Mutant Activity by Imidazole: Importance of Proximal Ligand Tethering. Newmyer, S. L., Sun, J., Loehr, T. M., and Ortiz de Montellano, P. R. (1996) Biochemistry 35, 12788 - 12795.  HTML

Protein Radicals in Myoglobin Dimerization and Myoglobin-Catalyzed Styrene Epoxidation. Tschirret-Guth, R. A. and Ortiz de Montellano, P. R. (1996) Arch. Biochem. Biophys. 335, 93-101. HTML PDF

Human Endothelial Nitric Oxide Synthase: Expression in Escherichia coli, Coexpression with Calmodulin, and Characterization. Rodríguez-Crespo, I. and Ortiz de Montellano, P. R. (1996) Arch. Biochem. Biophys. 336, 151-156. Abstract PDF

1995

Heme Oxygenase His-25->Ala Mutant: Replacement of the Proximal Histidine Iron Ligand by Exogenous Bases Restores Catalytic Activity. Wilks, A., Sun, J., Loehr, T. M., and Ortiz de Montellano, P. R. (1995) J. Am. Chem. Soc. 117, 2925-2926.

Expression and Characterization of Truncated Human Heme Oxygenase (hHO-1) and a Fusion Protein of hHO-1 with Cytochrome P450 Reductase. Wilks, A., Black, S. M., Miller, W. L., and Ortiz de Montellano, P. R. (1995) Biochemistry 34, 4421-4427.

Synthesis and Assignment of the Four Meso-Methylmesoporphyrin IX Regioisomers. Torpey, J. W. and Ortiz de Montellano, P. R. (1995) J. Org. Chem. 60, 2195-2199.

Computer-Assisted, Structure-Based Prediction of Substrates for Cytochrome P450cam. De Voss, J. J. and Ortiz de Montellano, P. R. (1995) J. Am. Chem. Soc. 117, 4185-4186.

Chloroperoxidase-Catalyzed Benzylic Hydroxylation. Miller, V. P. and Ortiz de Montellano, P. R. (1995) Arch. Biochem. Biophys. 319, 333-340.

Molecular Engineering of Horseradish Peroxidase: Thioanisole Sulfoxidation and Styrene Epoxidation by Phe-41 Leucine and Threonine Mutants. Ozaki, S. and Ortiz de Montellano, P. R. (1995) J. Am. Chem. Soc. 117, 7056-7064.

Neuronal Nitric Oxide Synthase: Expression in Escherichia coli, Irreversible Inhibition by Phenyldiazene, and Active Site Topology. Gerber, N. C. and Ortiz de Montellano, P. R. (1995) J. Biol. Chem. 270, 17791-17796.  Abstract, HTML

Horseradish Peroxidase Phe-172 -> Tyr Mutant: Sequential Formation of Compound I with a Porphyrin Radical Cation and a Protein Radical. Miller, V. P., Goodin, D. B., Friedman, A., and Ortiz de Montellano, P. R. (1995) J. Biol. Chem. 270, 18413-18419.  Abstract  HTML

Horseradish Peroxidase His-42->Ala , His-42 -> Val, and Phe-41->Ala Mutants: Histidine Catalysis and Control of Substrate Access to the Heme Iron. Newmyer, S. L. and Ortiz de Montellano, P. R. (1995) J. Biol. Chem. 270, 19430-19438.  Abstract HTML

Active Site Topology of Bovine Cholesterol Side-Chain Cleavage Cytochrome P450 (P450scc) and Evidence for Interaction of Tyrosine 94 with the Side-Chain of Cholesterol. Pikuleva, I. A., Mackman, R. L., Kagawa, N., Waterman, M. R., and Ortiz de Montellano, P. R. (1995) Arch. Biochem. Biophys. 322, 189-197.

Characterization of Rat Neuronal Nitric Oxide Synthase Expressed in Saccharomyces Cerevisiae. Black, S. M. and Ortiz de Montellano, P. R. (1995) DNA Cell Biol.. 14, 789-794.

1994

Lipoxygenase Activity of Myoglobin: Oxidation of Linoleic Acid by the Ferryl Oxygen and Rather than Protein Radical. Rao, S., Wilks, A., Hamberg, M., and Ortiz de Montellano, P. R. (1994) J. Biol. Chem. 269, 7210-7216. Abstract

Effects of 17-Octadecynoic Acid, a Suicide-Substrate Inhibitor of Cytochrome P450 Fatty Acid w-Hydroxylases, on Renal Function in Rats. Zou, A.-P., Ma, Y.-W., Sui, Z., Ortiz de Montellano, P. R., Clark, J. E., Masters, B. S. S., and Roman, R. J. (1994) J. Pharmacol. Exp. Therap. 268, 474-481. Abstract

Inhibition of Renal Vascular 20-HETE Production Impairs Autoregulation of Renal Blood Flow. Zou, A.-P., Imig, J. D., Kaldunski, M., Ortiz de Montellano, P. R., Sui, Z., and Roman, R. J. (1994) Am. J. Physiol. 266 (Renal Fluid Electrolyte Physiol. 35), F275-F282.

Haloperidol-Based Irreversible Inhibitors of the HIV-1 and HIV-2 Proteases. De Voss, J., Sui, Z., DeCamp, D. L., Salto, R., Babé, L. M., Craik, C. S., and Ortiz de Montellano, P. R. (1994) J. Med. Chem. 37, 665-673.

In Vitro Characterization of Non-Peptide Irreversible Inhibitors of HIV Proteases. Salto, R., Babé, L. M., Li, J., Rosé, J. R., Yu, Z., Burlingame, A., De Voss, J., Sui, Z., Ortiz de Montellano, P. R., and Craik, C. S. (1994) J. Biol. Chem. 269, 10691-10698. Abstract

Differential Roles of Glu318 and Thr319 in Cytochrome P450 1A2 Catalysis Supported by NADPH-Cytochrome P450 Reductase and tert-Butyl Hydroperoxide. Hiroya, K., Murakami, Y., Shimizu, T., Hatano, M., and Ortiz de Montellano, P. R. (1994) Arch. Biochem. Biophys. 310, 397-401.

Molecular Engineering of Horseradish Peroxidase. Highly Enantioselective Sulfoxidation of Aryl Alkyl Sulfides by the F41L Mutant. Ozaki, S. and Ortiz de Montellano, P. R. (1994) J. Am. Chem. Soc. 116, 4487-4488.

Proton NMR Investigation of Substrate-Bound Heme Oxygenase: Evidence for Electronic and Steric Contributions to Stereoselective Heme Cleavage. Hernández, G., Wilks, A., Paolesse, R., Smith, K. M., Ortiz de Montellano, P. R., and La Mar, G. N. (1994) Biochemistry 33, 6631-6641.

Cytochrome P450 Inhibitors Alter Afferent Arteriolar Responses to Elevations in Pressure. Imig, J. D., Zou, A.-I., Ortiz de Montellano, P. R., Sui, Z., and Roman, R. J. (1994) Am. J. Physiol. 266 (Heart Circ. Physiol. 35) H1879-H1885.

Endogenous P450 Metabolites of Arachidonic Acid Modulate Tubuloglomerular Feedback. Zou, A.-I., Imig, J. D., Ortiz de Montellano, P. R., Sui, Z., Falck, J. R., and Roman, R. J. (1994) Am. J. Physiol. 266 (Renal Fluid Electrolyte Physiol. 35) F934-F941.

Requirement of a Second Oxidation Equivalent for Ferryl-Oxygen Transfer to Styrene in the Epoxidation Catalyzed by Myoglobin-H2O2. Choe, Y. S., Rao, S. I., and Ortiz de Montellano, P. R. (1994) Arch. Biochem. Biophys. 314, 126-131.

Non-Peptide HIV Protease Inhibitors. Differential Introduction of Alkylamino Groups into the Two Aryl Rings of Haloperidol. Lee, K. H., McPhee, F., Craik, C. S., and Ortiz de Montellano, P. R. (1994) J. Org. Chem. 59, 6194-6199.

Relationship of Active Site Topology to Substrate Specificity for Cytochrome P450terp (CYP108). Fruetel, J. A., Mackman, R. L., Peterson, J. A., and Ortiz de Montellano, P. R. (1994) J. Biol. Chem. 269, 28815-28821.

Heme Oxygenase (HO-1): Evidence for Electrophilic Addition of Oxygen to the Porphyrin Ring in the Formation of a-meso-Hydroxyheme. Wilks, A., Torpey, J., and Ortiz de Montellano, P. R. (1994) J. Biol. Chem. 269, 29553-29556. Abstract

Identification of Histidine-25 as the Heme Ligand in Human Liver Heme Oxygenase, Sun, J., Loehr, T. M., Wilks, A., and Ortiz de Montellano, P. R. (1994) Biochemistry 33, 13734-13740.

Thioanisole Sulfoxidation by Cytochrome P450cam (CYP101): Experimental and Calculated Absolute Stereochemistries. Fruetel, J., Chang, Y.-T., Collins, J., Loew, G., and Ortiz de Montellano, P. R. (1994) J. Am. Chem. Soc. 116, 11643-11648.


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