Recent Publications
A microRNA derived from an apparent canonical biogenesis pathway regulates variant surface protein gene expression in Giardia lamblia. Saraiya AA, Li W, Wang CC. RNA (in revision)
Gene regulation in Giardia lamblia involves a putative microRNA derived from a small nucleolar RNA.  Li W, Saraiya AA, Wang CC. PLoS Negl Trop Dis. (in press)
A La autoantigen homologue is required for the internal ribosome entry site mediated translation of giardiavirus. Garlapati S, Saraiya AA, Wang CC. PLoS One. 2011 Mar 29;6(3):e18263.

Rab11 and actin cytoskeleton participate in Giardia lamblia encystation, guiding the specific vesicles to the cyst wall. Castillo-Romero A, Leon-Avila G, Wang CC, Perez Rangel A, Camacho Nuez M, Garcia Tovar C, Ayala-Sumuano JT, Luna-Arias JP, Hernandez JM. PLoS Negl Trop Dis. 4(6):e697

Polo-like kinase guides cytokinesis in Trypanosoma brucei through an indirect means. Li Z, Umeyama T, Li Z, Wang CC. Eukaryotic Cell. 9(5):705-16. Epub 2010 Mar 12

Giardiavirus internal ribosome entry site has an apparently unique mechanism of initiating translation. Garlapati S, Wang CC. PLoS One. 2009 Oct 14;4(10):e7435.
CRK9 contributes to regulation of mitosis and cytokinesis in the procyclic form of Trypanosoma brucei. Gourguechon S, Wang CC.Gourguechon S, Wang CC. BMC Cell Biol. 2009 Sep 21;10:68.
The Aurora Kinase in Trypanosoma brucei plays distinctive roles in metaphase-anaphase transition and cytokinetic initiation. Li Z, Umeyama T, Wang CC. PLoS Pathog. 2009 Sep;5(9):e1000575. Epub 2009 Sep 11.

Identification of a Bacterial-like HslVU Protease in the Mitochondria of Trypanosoma brucei and Its Role in Mitochondrial DNA Replication. Z. Li, ME Lindsay, SA Motyka, PT Englund and CC Wang. PLoS Pathogens 4(4), e1000048 (14 Apr 2008).
Identification of a Novel Chromosomal Passenger Complex and Its Unique Localization during Cytokinesis in Trypanosoma brucei. Z. Li, JH Lee, F Chu, AL Burlingame, A Gunzl and CC Wang. PLoS ONE 3(6), e2354 (11 Jun 2008).
Polo-like Kinase Is Expressed in S/G2/M-phase and Associated with FAZ in Both Procyclic and Bloodstream Trypanosoma brucei. T Umeyama and CC Wang. Eukaryotic Cell 7(9):1582-1590 (Sep 2008).
KMP-11, a Basal Body and Flagellar Protein, Is Required for Cell Division in Trypanosoma brucei. Z Li and CC Wang. Eukaryotic Cell 7(11):1941-50 (26 Sep 2008).
The Chromosomal Passenger Complex and a Mitotic Kinesin Interact with the Tousled-like Kinase in Trypanosoma brucei to Regulate Chromosome Segregation and Cytokinesis. Z Li, T Umeyama, and CC Wang. PLoS ONE 3(11):e3814 (26 Nov 2008).
snoRNA, a Novel Precursor of microRNA in Giardia lamblia. AA Saraiya and CC Wang. PLoS Pathogens 4(11):e1000224 (28 Nov 2008).

Centrin 1 Is Required for Organelle Segregation and Cytokinesis in Trypanosoma brucei. A Selvapandiyan, P Kumar, JC Morris, JL Salisbury, CC Wang and HL Nakhasi. Mol. Biol. Cell 18, 3290-3301 (2007).
Touseled-like Kinase in a Microbial Eukaryote Regulates Spindle Assembly and S-Phase Progression by Interacting with Aurora Kinase and Chromatin Assembly Factors. Z Li, S Gourguechon and CC Wang. J. Cell Sci. 120, 3883-3894 (2007).
Draft Genome Sequence of the Sexually Transmitted Pathogen Trichomonas vaginalis. JM Carlton, RP Hirt, JC Silva, AL Delcher, M Schatz, Q Zhao, JR Wortman, SL Bidwell, UC Alsmark, S Besteiro, T Sicheritz-Ponten, CJ Noel, JB Dacks, PG Foster, C Simillion, Y Van de Peer, D Miranda-Saavedra, GJ Barton, GD Westrop, S Muller, D Dessi, PL Fiori, Q Ren, I Paulsen, H Zhang, FD Bastida-Corcuera, A Simoes-Barbosa, MT Brown, RD Hayes, M Mukherjee, CY Okumura, R Schneider, AJ Smith, S Vanacova, M Villalvazo, BJ Haas, M Pertea, TV Feldblyum, TR Utterback, CL Shu, K Osoegawa, PJ de Jong, I Hrdy, L Horvathova, Z Zubacova, P Dolezal, SB Malik, JM Logsdon Jr, K Henze, A Gupta, CC Wang, RL Dunne, JA Upcroft, P Upcroft, O White, SL Salzberg, P Tang, CH Chiu, YS Lee, TM Embley, GH Coombs, JC Mottram, J Tachezy, CM Fraser-Liggett and PJ Johnson. Science 315, 207-212 (2007).
The Multiple Roles of Cyclin E1 in Controlling Cell Cycle Progression and Cellular Morphology of Trypanosoma brucei. S. Gourguechon, J.M. Savich and C.C. Wang. J. Mol. Biol. 368, 939-950 (2007).
Inhibition and Structure of Trichomonas vaginalis Purine Nucleoside Phosphorylase with Picomolar Transition State Analogues. A. Rinaldo-Mathis, C. Wing, M. Ghanem, H. Deng, P. Wu, A. Gupta, P.C. Tyler, G.B. Evans, R.H. Furneaux, S.C. Almo, C.C. Wang and V.L. Schramm. Biochemistry 46, 659-668 (2007)

An Aurora Kinase Homologue Is Involved in Regulating both Mitosis and Cytokinesis in Trypanosoma brucei. X Tu, P Kumar, Z Li and CC Wang. J. Biol. Chem. 281(14):9677-87 (Apr 7 2006)
Changing Roles of Aurora-B Kinase in Two Life Cycle Stages of Trypanosoma brucei. Z Li and CC Wang. Eukaryotic Cell 5, 1026-1035 (2006).
Okadaic Acid Overcomes the Blocked Cell Cycle Caused by Depleting Cdc2-related Kinases in Trypanosoma brucei. Z. Li, X. Tu and C.C. Wang. Exp. Cell Res. 312, 3504-3516 (2006)
A Likely Molecular Basis of the Susceptibility of Giardia lamblia Toward Oxygen. L Li and CC Wang. Mol. Microbiol. 59, 202-211 (2006)
Dissociation of Cytokinesis Initiation from Mitotic Control in a Eukaryote. P Kumar and CC Wang. Eukaryotic Cell 5, 92-102 (2006)

Pairwise Knockdowns of cdc2-Related Kinases (CRKs) in Trypanosoma brucei Identified the CRKs for G1/S and G2/M Transitions and Demonstrated Distinctive Cytokinetic Regulations between Two Developmental Stages of the Organism. Tu X, Wang CC. Eukaryot Cell. 2005 Apr;4(4):755-64.
Structural elements in the 5'-untranslated region of giardiavirus transcript essential for internal ribosome entry site-mediated translation initiation. Garlapati S, Wang CC. Eukaryot Cell 2005 Apr;4(4):742-54.
Identification of a subversive substrate of Trichomonas vaginalis purine nucleoside phosphorylase and the crystal structure of the enzyme-substrate complex. Zhang Y, Wang WH, Wu SW, Ealick SE, Wang CC. J. Biol. Chem. (2005) 280, 22318-22325
Coupling of posterior cytoskeletal morphogenesis to the G1/S transition in the Trypanosoma brucei cell cycle. Tu X, Wang CC. Mol Biol Cell. 2005 Jan;16(1):97-105.
Identification in the Ancient Protist Giardia lamblia of two eIF4E Homologues with Distinctive Functions. L Li and CC Wang. Eukaryotic Cell 4, 948-959 (2005).
Distinct Cytoskeletal Modulation and Regulation of G1/S Transition in the Two Life Stages of Trypanosoma brucei. X Tu, J Mancuso, WZ Cande and CC Wang. J. Cell Sci. 118, 4353-4364 (2005).
Depletion of APC/C subunit homolog APC1 or CDC27 of Trypanosoma brucei arrests the procyclic form in metaphase but the bloodstream form in anaphase. Kumar P, Wang CC. J. Biol. Chem. 280, 31783-31791 (2005).

The involvement of two cdc2-related kinases (CRKs) in Trypanosoma brucei cell cycle regulation and the distinctive stage-specific phenotypes caused by CRK3 depletion. Tu X, Wang CC. J Biol Chem. 2004 May 7; 279(19):20519-28. Epub 2004 Mar 08.
Capped mRNA with a single nucleotide leader is optimally translated in a primitive eukaryote, Giardia lamblia. Li L, Wang CC. J Biol Chem. 2004 Apr 9; 279(15):14656-64. Epub 2004 Jan 13.
Identification of a Novel Internal Ribosome Entry Site in Giardiavirus That Extends to Both Sides of the Initiation Codon. S Garlapati and CC Wang. J. Biol. Chem. 279, 3389-3397 (2004).

Differentiation of Trypanosoma brucei may be stage non-specific and does not require progression of cell cycle. Li Y, Li Z, Wang CC. Mol Microbiol.49(1):251-65 (2003 Jul).
Adenosine is the primary precursor of all purine nucleotides in Trichomonas vaginalis. Munagala NR, Wang CC. Mol Biochem Parasitol. 127(2):143-9 (2003 Apr 3).
A PHO80-like cyclin and a B-type cyclin control the cell cycle of the procyclic form of Trypanosoma brucei. Li Z, Wang CC. J. Biol. Chem 278(23):20652-8 (2003 Jun 6, Epub 2003 Mar 28).
Biochemical analysis of the 20 S proteasome of Trypanosoma brucei. Wang CC, Bozdech Z, Liu CL, Shipway A, Backes BJ, Harris JL, Bogyo M. J. Biol. Chem. 278(18):15800-8 (2003 May 2, Epub 2003 Feb 24).

An Easily Dissociated 26S Proteasome Catalyzes an Essential Ubiquitin-mediated Protein Degradation Pathway in Trypanosoma brucei. Z. Li, C.-B. Zou, Y. Yao, M.A. Hoyt, S. McDonough, Z.B. Mackey, P. Coffino and C.C. Wang. J. Biol. Chem. 277, 15486-15498 (2002).
Identification of an Essential Pseudoknot in the Downstream Putative Internal Ribosome Entry Site in Giardiavirus Transcript. S. Garlapati and C.C. Wang. RNA 8, 601-611 (2002).
The Pivotal Role of Guanine Phosphoribosyltransferase in Purine Salvage by Giardia lamblia. N. Munagala and C.C. Wang. Mol. Microbiol. 44, 1073-1079 (2002).
The Purine Nucleoside Phosphorylase from Trichomonas vaginalis is a Homologue of the Bacterial Enzyme. N. Munagala and C.C. Wang. Biochemistry 41, 10382-10389 (2002).
The Adenine Phosphoribosyltransferase from Giardia lamblia Has a Unique Reaction Mechanism and Unusual Substrate Binding Properties. A.E. Sarver and C.C. Wang. J. Biol. Chem.  277 (42):39973-39980 (2002).
Closed-site Complexes of Adenine Phosphoribosyltransferase from Giardia lamblia Reveal a Mechanism of Ribosyl Migration. W. Shi, A.E. Sarver, C.C. Wang, K.S.E. Tanaka, S.C. Almo and V.L. Schramm. J. Biol. Chem.  277 (42):39981-39988 (2002).
Functional Characterization of the ElevenNon-ATPase Subunit Proteins in the Trypanosome 19S Proteasome Regulatory Complex. Z. Li and C.C. Wang. J. Biol. Chem.   277 (42):42686-42693 (2002)

Role of the Flexible Loop in Hypoxanthine-Guanine-Xanthine Phosphoribosyltransferase from Tritrichomonas foetus in Enzyme Catalysis. N.R. Munagala, V. Basus and C.C. Wang. Biochemistry 40, 4303-4311 (2001)
Specific Secondary Structures in the Capsid Coding Region of Giardiavirus Transcript Are Required for Its Translation Initiation in Giardia lamblia. S. Garlapati, J. Chou and C.C. Wang. J. Mol. Biol. 308, 623-638 (2001).
Functional Assignment of the 20S Proteasome from T. brucei by Mass Spectrometry Using New Bioinformatics Tools. L. Huang, R.J. Jacob, S.C.-H. Pegg, M.A. Baldwin, C.C. Wang, A.L. Burlingame and P.C. Babbitt. J. Biol. Chem. 276, 28327-28339 (2001).
Virtual Screening of Combinatorial Libraries across a Gene Family, In Search of Inhibitors of Giardia lamblia Guanine Phosphoribosyltransferase. A.M. Aronov, N.R. Munagala, I.D. Kuntz and C.C. Wang. Antimicrob. Agents Chemother. 45, 2571-2576 (2001).
Structural Analysis of -1 Ribosomal Frameshift Elements in Giardivirus. L. Li, A.L. Wang and C.C. Wang. J. Virol. 75, 10621-10622 (2001).

Rational Design of Selective Submicromolar Inhibitors of Tritrichomonas foetus Hypoxanthine-Guanine-Xanthine Phosphoribosyltransferase. AM Aronov, NR Munagala, PR Ortiz de Montellano, ID Kuntz and CC Wang. Biochemistry 39, 4684-4691 (2000).
Inhibition of Pyruvate-Ferredoxin Oxidoreductase Gene Expression in Giardia lamblia by Virus-Mediated Hammerhead Ribozyme. M Dan, AL Wang and CC Wang. Mol. Microbiol. 447-456 (2000).
Role of Alcohol Dehydrogenase E (ADHE) in the Energy Metabolism of Giardia lamblia. M Dan and CC Wang.  Biochem. Parasitol. 109, 25-36 (2000).
Crystal Structures of Giardia lamblia Guanine Phosphoribosyltransferase at 1.75 Å.  W Shi, NR Munagala, CC Wang, CM Li, PC Tyler, RH Furneaux, C Grubmeyer, VL Schramm and SC Almo.   Biochemistry  39, 6781-6790 (2000).
The Putative Transcription Initiation Site in Giardiavirus Double-stranded RNA Genome.   D-C Yu, AL Wang and CC Wang.   Mol Biochem Parasitol  417-421 (2000).
Structural Basis for the Activation of 20S Proteasome by 11S Regulators.   FG Whitby, E Masters, L. Kramer, JR Knowlton, Y Yao, CC Wang and CP Hill. Nature   408, 115-120 (2000).
Converting the Guanine Phosphoribosyltransferase from Giardia lamblia to a Hypoxanthine-Guanine-Xanthine Phosphoribosyltransferase.   NR Munagala, AE Sarver   and CC Wang.   J. Biol Chem.   275, 37072-37077 (2000).
A Dual-Specificity Aminoacyl-tRNA Synthetase in the Deep-Rooted Eukaryote Giardia lamblia. S Bunjun, C Stathopoulos, D Graham, B Min, M Kitabatake, AL Wang, CC Wang, CP Vivares, LM Weiss and D Soll. Proc Natl Acad Sci  USA. 97, 12997-13002 (2000).
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