ATQA was developed by Daniel H. Wagner, Associates, and
provides users with confidence assessments for each basecall
derived from an electrophoretic chromatogram. Confidence scores are
computed by evaluating the quality of the fluorescent trace data.
ATQA assigns four confidence scores to each basecall; the first
three scores separately evaluate the likelihood of substitution,
insertion, and deletion errors. The fourth confidence score
corresponds to the combined probability of an error at the basecall
(i.e., a substitution or insertion error) or in the gap following
the basecall (a deletion error).
At present the Staden Package only uses the combined value.
Contact: Max Karlovitz
